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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Mapping PP1c and Its Inhibitor 2 Interactomes Reveals Conserved and Specific Networks in Asexual and Sexual Stages of Plasmodium
doi: 10.3390/ijms23031069
Figure Lengend Snippet: Workflow and study overview. Schizont and gametocyte stages were prepared from PbWT GFP, PbPP1c–mCherry, and PbI2–mCherry parasites. Immunoprecipitation was performed with anti-RFP antibody on 5 different biological replicates of schizonts and gametocytes extracts prepared from each parasite strain (PbWT, PbPP1c–mCherry, and PbI2–mCherry). The presence of mCherry-tagged PbPP1c and PbI2 was checked in the immunoprecipitated fractions (control wild-type vs. tagged proteins) before MS. Proteomic and statistical analyses (detailed in ) allowed for the identification of the interactome of PbPP1c and PbI2 in schizont and gametocyte stages that were further analyzed for GO enrichment, STRING networks, and comparative analyses.
Article Snippet: The presence of PbI2–mCherry protein was observed by Western blot with
Techniques: Immunoprecipitation